EVALUATION OF OXIDATIVE STRESS BIOMARKERS MDA AND SOD IN OCCUPATIONALLY EXPOSED ALUMINUM WORKERS IN ONITSHA, ANAMBRA STATE, NIGERIA

Authors

  • C.A Okechukwu Department of Human Biochemistry; College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria. Author
  • A.N Okpogba Department of Human Biochemistry; College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria Author
  • N.A Mbachu Department of Human Biochemistry; College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria Author
  • I.P Ezeugwunne Department of Human Biochemistry; College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Nigeria Author

DOI:

https://doi.org/10.61841/3qtqvw39

Keywords:

MDA, SOD, Oxidative Stress

Abstract

Aim: This research study was conducted to evaluate oxidative stress biomarkers using malondialdehyde (MDA) and superoxide dismutase (SOD) in aluminum workers exposed to occupational hazards.  

Research Method: The participants were recruited from an industrial area in Onitsha and divided into two groups: an exposure group composed of workers occupationally exposed to aluminum and a control group without exposure. The method of Gutteridge and Wilkins, (1982) was used for the estimation of malondialdehyde levels. SOD activity was measured according to the method as described by Misra and Fridovich, 1972.  Data were analyzed using SPSS version 25 (IBM, USA, 2018). Independent T-test, descriptive, statistics, and Spearman correlation were used to analyze the data.  

Results: The results showed a significant increase in MDA in the aluminum workers group (3.53±1.04) as compared to the control group (1.65±0.58) (p<0.005). The SOD levels were reduced in the aluminum workers (21.55±3.85) as compared with the control samples (24.12±3.54) (p<0.005), indicating an elevation of oxidative stress. The study results revealed a significant positive correlation between MDA levels, age and duration of exposure. The SOD levels revealed a nonsignificant negative correlation with age and a significant correlation with duration of exposure. 

Conclusion: The findings indicate that long-term occupational exposure to aluminum can lead to increased oxidative stress, as indicated by higher MDA levels and lower SOD levels

References

. Burg A., Silberstein T., Yardeni G., Tavor D., Blumenfeld J., Zilbermann I. and Saphier O. (2010). Role of radicals in the lipid peroxidation products of commercial infant milk formula. Jornal of agriculture and food chemistry. 58:2347–2350.

.Cordiano, R., Di Gioacchino, M., Mangifesta, R., Panzera, C., Gangemi, S. and Minciullo, P.L. (2023). Malondialdehyde as a Potential Oxidative Stress Marker for Allergy-Oriented Diseases: An Update. Molecules, 28, 5979.

. Gaweł, S., Wardas, M., Niedworok, E., and Wardas, P. (2004). Dialdehyd malonowy (MDA) jako wskaźnik procesów peroksydacji lipidów w organizmie [Malondialdehyde (MDA) as a lipid peroxidation marker]. Wiadomosci lekarskie . 57(9-10), 453–455.

. Ghezzi, P. (2020). Environmental risk factors and their footprints in vivo—A proposal for the classification of oxidative stress biomarkers. Redox Biology, 34, 101442

. Granger, D. N. and Kvietys, P. R. (2015). Reperfusion injury and reactive oxygen species: The evolution of a concept. Redox biology. 6: 524-551.

. Islam, M. N., Rauf, A., Fahad, F. I., Emran, T. B., Mitra, S., Olatunde, A., Shariati, M. A., Rebezov, M., Rengasamy, K. R. R., and Mubarak, M. S. (2022). Superoxide dismutase: An updated review on its health benefits and industrial applications. Critical Reviews in Food Science and Nutrition, 62(26), 7282-7300.

. Kangralkar, V.A., Patil, S.D. and Bandivadekar, R.M. (2010). Oxidative stress and diabetes: A review. international journal of applied pharmaceutical sciences and research. 1:38–45

. Koltuksuz, U., Uz, E., Ozen, S., Aydinç, M., Karaman, A. and Akyol, Ö. (2000). Plasma superoxide dismutase activity and malondialdehyde level correlate with the extent of acute appendicitis. Journal of pediatric surgery international. 16(8): 559–561.

. Rahal, A., Kumar, A., Singh, V., Yadav, B., Tiwari, R., Chakraborty, S. and Dhama, K. (2014). Oxidative stress, prooxidants, and antioxidants: the interplay. BioMed research international. 2014: 761-764.

. Sies, H. (2020). Oxidative stress: a concept in redox biology and medicine. Redox biology. 4: 180-183.

. Zarkovic, N. (2020). Roles and Functions of ROS and RNS in Cellular Physiology and Pathology. Cells. 9(3):767.

. Bergin, P., Leggett, A., Cardwell, C.R., Woodside, J.V., Thakkinstian, A., Maxwell, A.P. and McKay, G.J. (2021).The effects of vitamin E supplementation on malondialdehyde as a biomarker of oxidative stress in haemodialysis patients: A systematic review and meta-analysis. BMC Nephrology. 22:126-130.

.Misra, H.P. and Fridovich I.(1972). The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase. Journal of Biological Chemistry. 247:31-70.

. Gutteridge, J.M.C. and Wilkins, S.(1982).Copper-dependent hydroxyl radical damage to ascorbic acid: formation of a thiobarhuric acid reactive product. Federation of European Biomedical Societies Letters. 137 (2): 327-330

. Marnett L. J. (2002). Oxy radicals, lipid peroxidation and DNA damage. Toxicology, 181-182, 219–222. https://doi.org/10.1016/s0300-483x(02)00448-1

. Yokel, R. A., & McNamara, P. J. (2001). Aluminum toxicokinetics: an updated minireview. Pharmacology & Toxicology, 88(4), 159–167.

. Rahimzadeh, M. R., Rahimzadeh, M. R., Kazemi, S., Amiri, R. J., Pirzadeh, M., & Moghadamnia, A. A. (2022). Aluminum poisoning with emphasis on its mechanism and treatment of intoxication. Emergency Medicine International, 2022, 13.

. Bimla, N., & Priya, A. (2005). Oxidative damage following chronic aluminium exposure in adult and pup rat brains. Journal of Trace Elements in Medicine and Biology, 19, 203–208.

. Yuan, C. Y., Lee, Y. J., & Hsu, G. S. W. (2012). Aluminum overload increases oxidative stress in four functional brain areas of neonatal rats. Journal of Biomedical Sciences, 19, 51.

. Zhou, M., Yang, S., Wang, X., Zhang, X., Cen, X., Mu, G., Wang, D., Ma, J., Wang, B., & Chen, W. (2021). The association between urinary aluminum and lung function among an urban adult population: a repeated-measure longitudinal study. Chemosphere, 270, 129443.

. McClure, E. S., Vasudevan, P., DeBono, N., Robinson, W. R., Marshall, S. W., & Richardson, D. (2020). Cancer and noncancer mortality among aluminum smelting workers in Badin, North Carolina. American Journal of Industrial Medicine, 63(9), 755–765.

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Published

2024-03-12

How to Cite

Okechukwu, C., Okpogba , A., Mbachu, N., & Ezeugwunne , I. (2024). EVALUATION OF OXIDATIVE STRESS BIOMARKERS MDA AND SOD IN OCCUPATIONALLY EXPOSED ALUMINUM WORKERS IN ONITSHA, ANAMBRA STATE, NIGERIA. Journal of Advanced Research in Medical and Health Science (ISSN 2208-2425), 10(3), 80-86. https://doi.org/10.61841/3qtqvw39