LEVELS OF CATHEPSIN-D AND NESTIN IN BREAST CANCER PATIENTS

Khalid Bahram Arif

doi:10.53555/jp4agr96

Authors

Khalid Bahram Arif Department of Pathology, College of Medicine, University of Kirkuk, Kirkuk, Iraq Author

DOI:

https://doi.org/10.53555/jp4agr96

Keywords:

Tumor marker, Early and advanced stages, ELISA, Age, Kirkuk Oncology Center, Iraq

Abstract

Background: Breast cancer is the second most frequently cause of cancer deaths among women, therefore, new screening modalities could provide an extraordinary progress in understanding the mechanism of disease. In last decade, the role of Cathepsin-D (Cath-D) and Nestin which recently received attention as tumor markers, are remain unclear.

Aim: Measurement the levels of Cath-D and Nestin in breast cancer patients (BCPs) and estimating an association of each marker to stage of disease, early and advanced, and to age of study patients.

Materials and methods: An overall 48 adult women who attended to the Kirkuk Oncology Center (Kirkuk province, Iraq) and diagnosed clinically as BCPs; 24 at early stage and 24 at advanced stage, were selected to the present study that carried out from December (2022) to June (2023). The obtained sera were tested by specific ELISA kits.

Results: The quantitative findings of ELISA showed that the values of Cath-D and Nestin in BCPs were 283.997 18.12 pg/ml and 66.258 2.559 pg/ml, respectively. Moreover, significant higher values of Cath-D and Nestin were detected in advanced stage (395.683 14.737 pg/ml and 78.581 pg/ml, respectively) when compared to those of early stage (172.31 6.33 pg/ml and 53.934 2.36 pg/ml, respectively). According to age of study patients, insignificant variation (P 0.0) was seen in values of 40 and 41 years old for both Cath-D (305.319 31.55 pg/m and 270.027 21.81 pg/ml, respectively) and Nestin (67.357 2.81 and 64.532 3.85 pg/ml, respectively).

Conclusion: This represents the first Iraqi study determined Cath-D and Nestin in BCPs. This study demonstrates that these markers were provided additional information about the level of cancer activity in patients at early and advanced stages of disease.

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