MOLECULAR DETECTION OF SERRATIA MARCESCENS ISOLATED FROM DIFFERENT CLINICAL CASES IN WASIT PROVINCE, IRAQ
DOI:
https://doi.org/10.53555/cjp73776Keywords:
Serratia marcescens, Urinary tract infections, DNA extraction, 16S rRNA geneAbstract
Background: Although previously considered non-pathogenic (harmless saprophyte) Serratia marcescens is now recognized as an important opportunistic pathogen combining a propensity for healthcare-associated infection and antimicrobial resistance. The current study was aimed to detect and identify Serratia marcescens isolated from different clinical cases, using a variety of genotypic and phenotypic criteria
Materials and Methods: Totally, 207 different clinical samples collected from Al-Zahra Teaching Hospital, Wasit province, Iraq were included in this study. They were different specimens involving urine (n = 122), skin wound infections (n =59), and, respiratory system infection ( n= 26). All isolates wereidentified by traditional culture methods and biochemical tests and analysis profile Vitek® 2 compact system. Then, DNA extractions of all positive 18 isolates were carried out for confirmed with PCR by the amplification of 16S rRNA gene.
Results: The culture results were shown an overall 8.69% (18) positive isolates. (15 samples from patients with urinary tract infection,1 sample from patients with respiratory system infection, and 2 samples from patients with wound infection). The highest isolation rate from urine, and the incidence of Serratia marcescens was higher among females than males.
Conclusions: Serratia marcescens played an important role in urinary tract infections. Further studies are needed to determine the prevalence of pathogenic bacteria from the hospital environment and to detect virulence genes in these isolates.
References
Abbas HA and Hegazy WAH. (2020). Repurposing anti-diabetic drug "Sitagliptin" as a novel virulence attenuating agent in Serratia marcescens. PLoS One. 15(4): e0231625.
Abdullah, A. H., Nadhom, B. N., and Al-Ammiri, H. H. (2017). Isolation and Identification of Serratia marcescens from Bovine Mastitis infections in Iraq and their Susceptibility to Antibiotics. J Entomol Zool Stud, 5(2), 489-492.
Ahmed Abbas Hamza1, Mithal KA. Al-Hassani (2023) Biofilm Formation, Antimicrobial Resistance in Serratia marcescens Isolated from Different Clinical Cases. Journal of Population Therapeutics and Clinical Pharmacology Vol 30(7):e460–e465. S
Al-Daoodi, A.A.(2002). Diagnostic and physiological study on Serratia marcescens isolated from infected wound for patients bedriden in surgical wards. MSc. Thesis, College of Science, Mosul University, Mosul, Iraq.
Ali, M.R.(2012).Emergence and detection of AmpC gene by polymerase chain reaction from sepsis Serratia marcescens. Al-Mustansiriyah.J.Sci..23( 1): 39-54.
Ali,T. S.(2007). Antibiomicrobial Susceptibility Testing of Serratia Species Isolated from Hospitalized Patients in Two Hospitals in Al-Mosul, Iraq. Med J.; 41(2): 121-128.
Allen, J. L., Doidge, N. P., Bushell, R. N., Browning, G. F., and Marenda, M. S. (2022). Healthcare-associated infections caused by chlorhexidine-tolerant Serratia marcescens carrying a promiscuous IncHI2 multi-drug resistance plasmid in a veterinary hospital. PloS one, 17(3), e0264848.
Almaghrabi, M. K., Joseph, M. R., Assiry, M. M., and Hamid, M. E. (2018). Multidrug-resistant Acinetobacter baumannii: An emerging health threat in Aseer region, Kingdom of Saudi Arabia. Canadian Journal of Infectious Diseases and Medical Microbiology, 2018.
Barman S, Bhattacharya S S, Chandra M N. (2020). Serratia. Beneficial Microbes in Agro-Ecology. 27–36. doi:10.1016/b978-0-12-823414-3.00003
Bush K., Megan P., John L. Anne M. Q., James H. J., Ryan M. L., James S. L., and Deidre J.(2013). Detection systems for carbapenemase gene identification should include the SME serine carbapenemase. International J. Antimicrobiol Agents., 41:1-4.
Empel,J., Anna B., Elżbieta L., Agnieszka M., Janusz F., Ewa S., Waleria H., Marek G. and Beta-PL Study Group (2008). Molecular survey of β-lactamases conferring resistance to newer β-lactams in Enterobacteriaceae isolates from Polish hospitals. Antimicrob. Agents Chemother. 52:2449–2454.
Ferreira, C. M., and Serpa, S. (2018). Society 5.0 and social development. Management and Organizational Studies, 5(4), 26-31.
Gharban, H. A. (2022). Clinical and Serological Diagnosis of Bovine Hypodermosis in Wasit Province. Revista Electronica de Veterinaria, 23 (3), 457-466
Kljakić D, Milosavljević MZ, Jovanović M, Popović VČ, Raičević S. Serratia marcescens as a cause of unfavorable outcome in the twin pregnancy. Open Med (Wars). 2021;16(1):81-86.
Laupland, K.B., Parkins M.D., Gregon D.B., Church D., RossT., Pitout J.D. (2008). Population based laboratory surveillance for Serratia species isolates in a large Canadian health region. Eur. J.Clin. Mirobiol. Infect. Dis., 27: 89-95.
Mahlen, S.D. (2011). Serratia Infections: from Military Experiments to Current Practice.Clinical microbiologyreviews., 24(4): 755-791.
Marty, K.B.,Williams C.L., Guynn L.J., Bendik M.J. and Blanke S.R. (2002). Characterization of a cytotoxic factor in culture filtrates of Serratia marcescens. Infect. Immun.,70(3): 1121-1128.
Pérez-Viso, B., Aracil-Gisbert, S., Coque, T. M., Del Campo, R., Ruiz-Garbajosa, P., and Cantón, R. (2021). Evaluation of CHROMagar™-Serratia agar, a new chromogenic medium for the detection and isolation of Serratia marcescens. European Journal of Clinical Microbiology and Infectious Diseases, 40(12), 2593-2596.
Poole, K. E. I. T. H., and Braun, V. O. L. K. M. A. R. (1988). Influence of growth temperature and lipopolysaccharide on hemolytic activity of Serratia marcescens. Journal of bacteriology, 170(11), 5146-5152.
Prado, L. C. D. S., Giacchetto Felice, A., Rodrigues, T. C. V., Tiwari, S., Andrade, B. S., Kato, R. B., ... and Soares, S. D. C. (2021). New putative therapeutic targets against Serratia marcescens using reverse vaccinology and subtractive genomics. Journal of Biomolecular Structure and Dynamics, 1-16. Quinn PJ, Markey BK,
Carter ME, Donnelly WJ and Leonard FC 2004. . 1 Ed., Veterinary Microbiology and Microbial DiseasesstBlackwell Science Ltd.: 225.),
Sambrook, J., and Russell, D. W. (2006). Differential Display-PCR. Cold Spring Harbor Protocols, 2006(1), pdb-prot3844.
Shaimaa Obaid Hasson , Adnan Hamad Al-Hamadani , Ibtisam Habeeb Al- Azawi (2018) Occurrence of Biofilm Formation in Serratia fonticola and Pantoea sp. Isolates among Urinary Catheterized Patients. Nano Biomed. Eng., 2018, Vol. 10, Iss. 3. p295-304)
Stella, N. A., Romanowski, E. G., Brothers, K. M., Calvario, R. C., and Shanks, R. M. (2022). IgaA protein, GumB, has a global impact on the transcriptome and surface proteome of Serratia marcescens. Infection and Immunity, 90(11), e00399-22.
Sutherland K, Porter J, Turner J, Thomas B, Looney E, Luna T, Meyers M, Futch J and Lipp E 2010. Human sewage identified as likely source of white pox disease of the threatened Caribbean elkhorn coral, Acropora palmata. Environmental Microbiology 12: 1122-1131
Tariq, A. L. and Prabakaran J.(2011). Isolation and molecular characterization of UTI associated Serratia marcescens TW1 from urine specimens. J. Microbiol. Antimicrobials., 3(6):130-135.
Yang, H., Cheng J., Hu L., Zhu Y. and Li J.(2012). Mechanisms of antimicrobial resistance in Serratia marcescens . African J.Microbiol. Research., 6(21): 4427-4437.
Ye, X., and Lei, B. (2023). The current status and trends of DNA extraction. BioEssays, 2200242.
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